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Targeting and tracing antigens in live cells with fluorescent nanobodies

Item Type:Article
Title:Targeting and tracing antigens in live cells with fluorescent nanobodies
Creators Name:Rothbauer, U. and Zolghadr, K. and Tillib, S. and Nowak, D. and Schermelleh, L. and Gahl, A. and Backmann, N. and Conrath, K. and Muyldermans, S. and Cardoso, M.C. and Leonhardt, H.
Abstract:We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion.
Keywords:Antibodies, Antigen-Antibody Reactions, Antigens, Camels, Epitopes, Green Fluorescent Proteins, Immunoglobulin Heavy Chains, Mitosis, S Phase, Animals
Source:Nature Methods
ISSN:1548-7091
Publisher:Nature Publishing Group (U.K.)
Volume:3
Number:11
Page Range:887-889
Date:November 2006
Official Publication:https://doi.org/10.1038/nmeth953
PubMed:View item in PubMed

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