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Targeting and tracing antigens in live cells with fluorescent nanobodies

Official URL:https://doi.org/10.1038/nmeth953
PubMed:View item in PubMed
Creators Name:Rothbauer, U. and Zolghadr, K. and Tillib, S. and Nowak, D. and Schermelleh, L. and Gahl, A. and Backmann, N. and Conrath, K. and Muyldermans, S. and Cardoso, M.C. and Leonhardt, H.
Journal Title:Nature Methods
Journal Abbreviation:Nat Methods
Volume:3
Number:11
Page Range:887-889
Date:November 2006
Keywords:Antibodies, Antigen-Antibody Reactions, Antigens, Camels, Epitopes, Green Fluorescent Proteins, Immunoglobulin Heavy Chains, Mitosis, S Phase, Animals
Abstract:We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion.
ISSN:1548-7091
Publisher:Nature Publishing Group (U.K.)
Item Type:Article

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