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First steps towards effective methods in exploiting high-throughput technologies for the determination of human protein structures of high biomedical value

Item Type:Article
Title:First steps towards effective methods in exploiting high-throughput technologies for the determination of human protein structures of high biomedical value
Creators Name:Banci, L. and Bertini, I. and Cusack, S. and de Jong, R.N. and Heinemann, U. and Jones, E.Y. and Kozielski, F. and Maskos, K. and Messerschmidt, A. and Owens, R. and Perrakis, A. and Poterszman, A. and Schneider, G. and Siebold, C. and Silman, I. and Sixma, T. and Stewart-Jones, G. and Sussman, J.L. and Thierry, J.C. and Moras, D.
Abstract:The EC 'Structural Proteomics In Europe' contract is aimed specifically at the atomic resolution structure determination of human protein targets closely linked to health, with a focus on cancer (kinesins, kinases, proteins from the ubiquitin pathway), neurological development and neurodegenerative diseases and immune recognition. Despite the challenging nature of the analysis of such targets, approximately 170 structures have been determined to date. Here, the impact of high-throughput technologies, such as parallel expression of multiple constructs, the use of standardized refolding protocols and optimized crystallization screens or the use of mass spectrometry to assist sample preparation, on the structural biology of mammalian protein targets is illustrated through selected examples.
Keywords:Eukaryotic Cells, Gene Expression, Genetic Research, Immune System, Mass Spectrometry, Neoplasms, Nervous System Diseases, Proteins, Proteomics, Animals
Source:Acta Crystallographica Section D
ISSN:0907-4449
Publisher:International Union of Crystallography (U.K.)
Volume:62
Number:Pt. 10
Page Range:1208-1217
Date:October 2006
Official Publication:https://doi.org/10.1107/S0907444906029350
PubMed:View item in PubMed

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