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[Effect of cell surface sialic acid and their linkages on adhesion of mammary carcinoma cells]

Item Type:Article
Title:[Effect of cell surface sialic acid and their linkages on adhesion of mammary carcinoma cells]
Creators Name:Wang, X.Y. and Lin, S.Q. and Li, J.W. and Kemmner, W. and Ding, Y.Q.
Abstract:OBJECTIVE: To investigate the effect of cell surface sialic acid and its linkage on the cell-cell and cell-matrix adhesion of mammary carcinoma cells MD-MB-435. METHODS: MD-MB-435 cells were sense-transfected with ST6Gal I cDNA or antisense-transfected with part of the ST6Gal I sequence inserted in pcDNA 3.1 vector, with mock transfection with pcDNA3.1 vector as the control. The cell surface alpha2, 6-linked sialylation was determined by fluorescence-activated cell sorting (FACS) using lectin SNA (Sambucus nigra agglutinin specific to alpha2, 6-linked sialic acid on N-linked glycoprotein). A significantly increased alpha2, 6-sialylation subclone in sense-tranfectants and a decreased alpha2, 6-sialylation subclone in antisense-transfectants were selected for further examination of cell-cell and cell-matrix (collagen IV) adhesion. The transfectants were also treated with sialidase to compare the capacity of cell adhesion affected by cell surface sialylation. RESULTS: Sense-transfection subclone showed a reduced cell-cell aggregation but enhanced cell-matrix adhesion. In contrast, the antisense-transfection subclone exhibited increased cell-cell aggregation and decreased cell-matrix adhesion. After treatment with sialidase, the cell-matrix adhesion of all the transfectants and the parental MDA-MB-435 cells were significantly reduced to the level of 31%-57% of untreated cells. CONCLUSION: Cell surface sialic acid and alpha2, 6-linked sialylation play an important role in cell-cell and cell-matrix adhesion of mammary carcinoma cell MDA-MB-435.
Keywords:Breast Neoplasms, Sialyltransferase, Extracellular matrix, Cell adhesion, Metastasis
Source:Journal of Southern Medical University
ISSN:1673-4254
Publisher:Southern Medical University (China)
Volume:26
Page Range:742-746
Date:June 2006
PubMed:View item in PubMed

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