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Detection, isolation and partial characterization of an immunostimulating glycoprotein from Rhodococcus fascians

Item Type:Article
Title:Detection, isolation and partial characterization of an immunostimulating glycoprotein from Rhodococcus fascians
Creators Name:Butschak, G. and Karsten, U. and Schelhaas, U. and Ott, H. and Emmendoerffer, A. and Niemeyer, B. and Helmholz, H. and Goletz, S.
Abstract:In a search for novel immunostimulating substances we detected that culture supernatants of the gram-positive phytopathogenic bacterium, Rhodococcus fascians, were able to induce cytokine release (TNFalpha) from mouse peritoneal macrophages. Monoclonal antibodies were generated against the active principle, and were employed for its isolation and partial characterization as a high molecular (MW>100 kDa) glycoprotein. In addition, methods practicable for its biotechnological preparation and several ELISA variants for its determination were developed.
Keywords:Immunostimulation, TNFalpha, Rhodococcus, Glycoconjugate, Immunotherapy, Animals, Mice
Source:International Immunopharmacology
ISSN:1567-5769
Publisher:Elsevier (The Netherlands)
Volume:6
Number:9
Page Range:1441-1450
Date:September 2006
Official Publication:https://doi.org/10.1016/j.intimp.2006.04.010
PubMed:View item in PubMed

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