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Direct observation of single protein molecules in aqueous solution

Item Type:Article
Title:Direct observation of single protein molecules in aqueous solution
Creators Name:Gruenwald, D., Hoekstra, A., Dange, T., Buschmann, V. and Kubitscheck, U.
Abstract:(Figure presented) Real-time visualisation and tracking: Fluorescent labeled protein molecules are tracked using high-speed imaging systems. The figure shows the fluorescence signal from single quantum dots integrated over 2.7 ms. Diffusion coefficients deduced from single-molecule traces are consistent with those determined independently by fluorescence correlated spectroscopy, demonstrating in differnt distances to the focal plane the effectiveness of the technique.
Keywords:Fluorescent Probes, Fluorescence Spectroscopy, Imaging Agents, Quantum Dots, Single-Molecule Studies
Source:Chemphyschem
ISSN:1439-4235
Publisher:Wiley-VCH Verlag GmBh
Volume:7
Number:4
Page Range:812-815
Date:10 April 2006
Official Publication:https://doi.org/10.1002/cphc.200500632
PubMed:View item in PubMed

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