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Mn2+-DNA interactions in aqueous systems: A Raman spectroscopic study

Item Type:Review
Title:Mn2+-DNA interactions in aqueous systems: A Raman spectroscopic study
Creators Name:Muntean, C.M. and Misselwitz, R. and Dostal, L. and Welfle, H.
Abstract:Interaction of natural calf thymus DNA with Mn2+ ions was studied by means of Raman spectroscopy. Spectra of DNA in 10 mM Na-cacodylate buffer, pH 6.2, 10 mM NaCl and in buffer containing Mn2+ ions were measured at room temperature. Mn2+ concentrations varied between 0 and 0.6 M. DNA backbone conformational changes and DNA denaturation were not observed in the concentration range 0 and 0.5 M, however, DNA condensation was observed at a critical concentration of 100 mM Mn2+ that prevented the measurement of Raman spectra. Binding of Mn2+ to the charged phosphate groups of DNA is indicated in the spectra. A high affinity of Mn2+ for guanine N7 was obvious, and binding to adenine was barely suggested.
Keywords:Mn2+ - DNA interaction, Raman spectroscopy, Difference spectra
Publisher:Advanstar Communications
Page Range:29-35
Date:January 2006
Official Publication:https://doi.org/10.1155/2006/429147

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