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A structural role for ATP in the formation and stability of the human origin recognition complex

Item Type:Article
Title:A structural role for ATP in the formation and stability of the human origin recognition complex
Creators Name:Ranjan, A. and Gossen, M.
Abstract:The locally restricted recruitment of the multisubunit origin recognition complex (ORC) to eukaryotic chromosomes defines the position of origins of DNA replication. In budding yeast and metazoans the DNA binding activity of ORC is stimulated by ATP and requires an AAA+-type nucleotide binding domain in the largest subunit. Little else is known about the mechanisms behind the ATP requirement for ORC in its initiator function and, specifically, the relevance of nucleotide binding domains present on other subunits. Here we show that ATP is required for specific subunit interactions in the human ORC, with the Orc4 subunit playing a critical role in this dynamic process. ATP is essential for the maintenance of ORC integrity and facilitates complex formation. Thus, besides its previously identified role in DNA binding, ATP serves also as a structural cofactor for human ORC.
Keywords:AAA+ Protein, DNA Replication, Prereplicative Complex, Animals
Source:Proceedings of the National Academy of Sciences of the United States of America
ISSN:0027-8424
Publisher:National Academy of Sciences (U.S.A.)
Volume:103
Number:13
Page Range:4864-4869
Date:28 March 2006
Official Publication:https://doi.org/10.1073/pnas.0510305103
PubMed:View item in PubMed

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