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Expression profiling of uniparental mouse embryos is inefficient in identifying novel imprinted genes

Item Type:Article
Title:Expression profiling of uniparental mouse embryos is inefficient in identifying novel imprinted genes
Creators Name:Ruf, N. and Duenzinger, U. and Brinckmann, A. and Haaf, T. and Nuernberg, P. and Zechner, U.
Abstract:Imprinted genes are expressed from only one allele in a parent-of-origin-specific manner. We here describe a systematic approach to identify novel imprinted genes using quantification of allele-specific expression by Pyrosequencing, a highly accurate method to detect allele-specific expression differences. Sixty-eight candidate imprinted transcripts mapping to known imprinted chromosomal regions were selected from a recent expression profiling study of uniparental mouse embryos and analyzed. Three novel imprinted transcripts encoding putative non-protein-coding RNAs were identified on the basis of parent-of-origin-specific monoallelic expression in E11.5 (C57BL/6 x Cast/Ei)F1 and informative (C57BL/6 x Cast/Ei) x C57BL/6 backcross embryos. In addition, four transcripts with preferential expression of a strain-specific allele were found. Intriguingly, a vast majority of the analyzed transcripts showed no imprinting-associated expression in F1 embryos. These data strengthen the view that a large fraction of nonimprinted genes is differentially expressed between parthenogenetic and androgenetic embryos and question the efficiency of expression profiling of uniparental embryos to identify novel imprinted genes.
Keywords:Genomic Imprinting, Gene Expression Profiling, Uniparental Embryo, Pyrosequencing, Animals, Mice
Publisher:Academic Press
Page Range:509-519
Date:April 2006
Official Publication:https://doi.org/10.1016/j.ygeno.2005.12.007
PubMed:View item in PubMed

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