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Proteome survey reveals modularity of the yeast cell machinery

Item Type:Article
Title:Proteome survey reveals modularity of the yeast cell machinery
Creators Name:Gavin, A.C. and Aloy, P. and Grandi, P. and Krause, R. and Boesche, M. and Marzioch, M. and Rau, C. and Jensen, L.J. and Bastuck, S. and Duempelfeld, B. and Edelmann, A. and Heurtier, M.A. and Hoffman, V. and Hoefert, C. and Klein, K. and Hudak, M. and Michon, A.M. and Schelder, M. and Schirle, M. and Remor, M. and Rudi, T. and Hooper, S. and Bauer, A. and Bouwmeester, T. and Casari, G. and Drewes, G. and Neubauer, G. and Rick, J.M. and Kuster, B. and Bork, P. and Russell, R.B. and Superti-Furga, G.
Abstract:Protein complexes are key molecular entities that integrate multiple gene products to perform cellular functions. Here we report the first genome-wide screen for complexes in an organism, budding yeast, using affinity purification and mass spectrometry. Through systematic tagging of open reading frames (ORFs), the majority of complexes were purified several times, suggesting screen saturation. The richness of the data set enabled a de novo characterization of the composition and organization of the cellular machinery. The ensemble of cellular proteins partitions into 491 complexes, of which 257 are novel, that differentially combine with additional attachment proteins or protein modules to enable a diversification of potential functions. Support for this modular organization of the proteome comes from integration with available data on expression, localization, function, evolutionary conservation, protein structure and binary interactions. This study provides the largest collection of physically determined eukaryotic cellular machines so far and a platform for biological data integration and modelling.
Keywords:Fungal Genome, Multiprotein Complexes, Open Reading Frames, Phenotype, Proteome, Proteomics, Saccharomyces cerevisiae
Publisher:Nature Publishing Group
Page Range:631-636
Date:30 March 2006
Official Publication:https://doi.org/10.1038/nature04532
PubMed:View item in PubMed

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