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Inhibition of hepatic chylomicron remnant uptake by gene transfer of a receptor antagonist

Item Type:Article
Title:Inhibition of hepatic chylomicron remnant uptake by gene transfer of a receptor antagonist
Creators Name:Willnow, T.E. and Sheng, Z. and Ishibashi, S. and Herz, J.
Abstract:The low density lipoprotein receptor-related protein (LRP) has been proposed to mediate in concert with the LDL receptor (LDLR) the uptake of dietary lipoproteins into the hepatocytes. This hypothesis was tested by transient inactivation of LRP in vivo. Receptor-associated protein (RAP), a dominant negative regulator of LRP function, was transferred by an adenoviral vector to the livers of mice lacking LDLR (LDLR-/-). The inactivation of LRP by RAP was associated with a marked accumulation of chylomicron remnants in LDLR-/- mice and to a lesser degree in normal mice, suggesting that both LDLR and LRP are involved in remnant clearance.
Keywords:Adenoviridae, Apolipoprotein B-48, Apolipoproteins B, Carrier Proteins, Cholesterol, Chylomicrons, Gene Transfer Techniques, Genetic Vectors, Glycoproteins, LDL-Receptor Related Protein 1, LDL-Receptor Related Protein-Associated Protein, Liver, Inbred C57BL Mice, Immunologic Receptors, LDL Receptors, Triglycerides, alpha-Macroglobulins/metabolism, Animals, Mice
Publisher:American Association for the Advancement of Science
Page Range:1471-1474
Date:3 June 1994
Official Publication:https://doi.org/10.1126/science.7515194
PubMed:View item in PubMed

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