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Classical Hodgkin lymphoma is characterized by high constitutive expression of activating transcription factor 3 (ATF3) which promotes viability of Hodgkin/Reed-Sternberg cells

Official URL:https://doi.org/10.1182/blood-2005-07-2694
PubMed:View item in PubMed
Creators Name:Janz, M. and Hummel, M. and Truss, M. and Wollert-Wulf, B. and Mathas, S. and Joehrens, K. and Hagemeier, C. and Bommert, K. and Stein, H. and Doerken, B. and Bargou, R.C.
Journal Title:Blood
Journal Abbreviation:Blood
Page Range:2536-2539
Date:15 March 2006
Keywords:Activating Transcription Factor 3, Cell Proliferation, Cell Survival, Gene Expression Profiling, Hodgkin Disease, Neoplastic Gene Expression Regulation, Oligonucleotide Array Sequence Analysis, Reed-Sternberg Cells, Small Interfering RNA, Tumor Cell Line
Abstract:Hodgkin/Reed-Sternberg (HRS) cells of classical Hodgkin lymphoma (cHL) display unique characteristics that discriminate cHL from other B-cell lymphomas and normal B cells. Therefore, comparative gene expression profiling of Hodgkin and non-Hodgkin B cells could lead to the identification of candidate genes that are critical for the pathogenesis of cHL. We performed microarray analysis of Hodgkin and non-Hodgkin cell lines and identified activating transcription factor 3 (ATF3), a member of the cyclic AMP response element binding protein (CREB)/ATF family, as a differentially expressed candidate gene. Extensive analysis of a large panel of cell lines, primary tumor samples, and normal tissues revealed that high expression ofATF3 is found in nearly all cases of cHL and is almost exclusively restricted to it. Selective knock-down of ATF3 by RNA interference suppressed proliferation and strongly reduced viability of Hodgkin cells. Thus, overexpression of ATF3 is a molecular hallmark of cHL that contributes to the malignant growth of HRS cells. © 2006 by The American Society of Hematology.
Publisher:American Society of Hematology (U.S.A.)
Item Type:Article

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