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The ratio between dendritic cells and T cells determines the outcome of their encounter: Proliferation versus deletion

Item Type:Article
Title:The ratio between dendritic cells and T cells determines the outcome of their encounter: Proliferation versus deletion
Creators Name:Hoepken, U.E. and Lehmann, I. and Droese, J. and Lipp, M. and Schueler, T. and Rehm, A.
Abstract:Dendritic cells (DC) either induce T cell tolerance or contribute to the initiation and modulation of T and B cell responses. Since many of the variables determining the thresholds of naive T cell priming were defined in vitro using a homogeneously matured DC population, we here focused on partially mature DC which might reflect the occurrence of tumor-infiltrating and thymic DC. To predict how those DC regulate the induction of antigen-specific T cell proliferation and T cell tolerance, we co-cultured ovalbumin-pulsed murine DC at different ratios with antigen-specific DO11.10 transgenic T cells. Whereas partially mature DC at a DC/T cell ratio of 1: 10 supported proliferation, a DC/T cell ratio of 1 : 2 induced proliferation arrest in naive CD4+ T cells. The acquisition of the NK cell inhibitory markers NKI.I and KLRG on T cells exposed to high numbers of DC suggests a role for these molecules in the protection of antigen-responsive T cells from exhaustion by overstimulation. Mechanistically, abortive T cell proliferation upon encounter of high numbers of partially mature DC is caused by an apoptosis-related pathway, suggesting that excessive antigen density without sufficient costimulation results in activation-induced cell death.
Keywords:Activation-induced cell death, Apoptosis, Dendritic cells, T cell tolerance, Animals, Mice
Source:European Journal of Immunology
ISSN:0014-2980
Publisher:Wiley (U.S.A.)
Volume:35
Number:10
Page Range:2851-2863
Date:23 September 2005
Official Publication:https://doi.org/10.1002/eji.200526298
PubMed:View item in PubMed

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