![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
| Item Type: | Article |
|---|---|
| Title: | The ratio between dendritic cells and T cells determines the outcome of their encounter: Proliferation versus deletion |
| Creators Name: | Höpken, U.E., Lehmann, I., Droese, J., Lipp, M., Schueler, T. and Rehm, A. |
| Abstract: | Dendritic cells (DC) either induce T cell tolerance or contribute to the initiation and modulation of T and B cell responses. Since many of the variables determining the thresholds of naive T cell priming were defined in vitro using a homogeneously matured DC population, we here focused on partially mature DC which might reflect the occurrence of tumor-infiltrating and thymic DC. To predict how those DC regulate the induction of antigen-specific T cell proliferation and T cell tolerance, we co-cultured ovalbumin-pulsed murine DC at different ratios with antigen-specific DO11.10 transgenic T cells. Whereas partially mature DC at a DC/T cell ratio of 1: 10 supported proliferation, a DC/T cell ratio of 1 : 2 induced proliferation arrest in naive CD4+ T cells. The acquisition of the NK cell inhibitory markers NKI.I and KLRG on T cells exposed to high numbers of DC suggests a role for these molecules in the protection of antigen-responsive T cells from exhaustion by overstimulation. Mechanistically, abortive T cell proliferation upon encounter of high numbers of partially mature DC is caused by an apoptosis-related pathway, suggesting that excessive antigen density without sufficient costimulation results in activation-induced cell death. |
| Keywords: | Activation-induced cell death, Apoptosis, Dendritic cells, T cell tolerance, Animals, Mice |
| Source: | European Journal of Immunology |
| ISSN: | 0014-2980 |
| Publisher: | Wiley |
| Volume: | 35 |
| Number: | 10 |
| Page Range: | 2851-2863 |
| Date: | 23 September 2005 |
| Official Publication: | https://doi.org/10.1002/eji.200526298 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
