Activation of AP-1 through reactive oxygen species by angiotensin II in rat cardiomyocytes

Item Type:	Article
Title:	Activation of AP-1 through reactive oxygen species by angiotensin II in rat cardiomyocytes
Creators Name:	Wu, S. and Gao, J. and Ohlemeyer, C. and Roos, D. and Niessen, H. and Koettgen, E. and Gessner, R.
Abstract:	Cardiovascular pathogenesis induced by angiotensin II (Ang-II) is a complex process often connected to oxidative stress. In the present study we show that, 4 h after addition, Ang-II induces a four- to fivefold increase in AP-1 activity in cultured neonatal rat cardiomyocytes and that the intracellular level of reactive oxygen species (ROS) correlates with the extent of AP-1 binding activity. Ang-II stimulated ROS generation in rat cardiomyocytes in a dose- and time-dependent manner. These effects of Ang-II were suppressed by the Ang-II receptor type I (AT 1) inhibitor CV-11974 as well as by the antioxidants diphenylene iodonium (DPI) and N-acetyl-l-cysteine (NAC), but not by AT 2 antagonist PD 122319. Furthermore, Ang-II induced a two- to threefold increase in protein synthesis and cell size during 12-24 h, which could be inhibited by CV-11974 as well as by DPI and NAC. Because the rat cardiomyocytes strongly expressed gp91 phox, this suggests that ROS generated in a gp91-containing NADPH oxidase are involved in signal transduction leading to AP-1 activation. Together, these findings indicate that Ang-II elicits the activation of the redox-sensitive AP-1 via ROS through AT 1, resulting in effects on cardiomyocyte function such as hypertrophy
Keywords:	Angiotensin II, AP-1, Cardiomyocyte, Free radicals, NAD(P)H oxidase components, ROS
Source:	Free Radical Biology and Medicine
ISSN:	0891-5849
Publisher:	Elsevier
Volume:	39
Number:	12
Page Range:	1601-1610
Date:	24 August 2005
Official Publication:	https://doi.org/10.1016/j.freeradbiomed.2005.08.006
PubMed:	View item in PubMed

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