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A human protein-protein interaction network: a resource for annotating the proteome

Item Type:Article
Title:A human protein-protein interaction network: a resource for annotating the proteome
Creators Name:Stelzl, U. and Worm, U. and Lalowski, M. and Haenig, C. and Brembeck, F.H. and Goehler, H. and Stroedicke, M. and Zenkner, M. and Schoenherr, A. and Koeppen, S. and Timm, J. and Mintzlaff, S. and Abraham, C. and Bock, N. and Kietzmann, S. and Goedde, A. and Toksoez, E. and Droege, A. and Krobitsch, S. and Korn, B. and Birchmeier, W. and Lehrach, H. and Wanker, E.E.
Abstract:Protein-protein interaction maps provide a valuable framework for a better understanding of the functional organization of the proteome. To detect interacting pairs of human proteins systematically, a protein matrix of 4456 baits and 5632 preys was screened by automated yeast two-hybrid (Y2H) interaction mating. We identified 3186 mostly novel interactions among 1705 proteins, resulting in a large, highly connected network. Independent pull-down and co-immunoprecipitation assays validated the overall quality of the Y2H interactions. Using topological and GO criteria, a scoring system was developed to define 911 high-confidence interactions among 401 proteins. Furthermore, the network was searched for interactions linking uncharacterized gene products and human disease proteins to regulatory cellular pathways. Two novel Axin-1 interactions were validated experimentally, characterizing ANP32A and CRMP1 as modulators of Wnt signaling. Systematic human protein interaction screens can lead to a more comprehensive understanding of protein function and cellular processes.
Keywords:Databases as Topic, Intracellular Signaling Peptides and Proteins, Molecular Models, Nerve Tissue Proteins, Protein Binding, Proteins, Proteomics, Repressor Proteins, Two-Hybrid System Techniques
Publisher:Cell Press
Page Range:957-968
Date:23 September 2005
Additional Information:The original publication is available at www.sciencedirect.com
Official Publication:https://doi.org/10.1016/j.cell.2005.08.029
PubMed:View item in PubMed

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