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Restricted mobility of Dnmt1 in preimplantation embryos: implications for epigenetic reprogramming

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Item Type:Article
Title:Restricted mobility of Dnmt1 in preimplantation embryos: implications for epigenetic reprogramming
Creators Name:Grohmann, M. and Spada, F. and Schermelleh, L. and Alenina, N. and Bader, M. and Cardoso, M.C. and Leonhardt, H.
Abstract:BACKGROUND: Mouse preimplantation development is characterized by both active and passive genomic demethylation. A short isoform of the prevalent maintenance DNA methyltransferase (Dnmt1S) is found in the cytoplasm of preimplantation embryos and transiently enters the nucleus only at the 8-cell stage. RESULTS: Using GFP fusions we show that both the long and short isoforms of Dnmt1 localize to the nucleus of somatic cells and the cytoplasm of preimplantation embryos and that these subcellular localization properties are independent of phosphorylation. Importantly, photobleaching techniques and salt extraction revealed that Dnmt1S has a very restricted mobility in the cytoplasm, while it is highly mobile in the nucleus of preimplantation embryos. CONCLUSION: The restricted mobility of Dnmt1S limits its access to DNA and likely contributes to passive demethylation and epigenetic reprogramming during preimplantation development.
Keywords:Blastocyst, Cell Nucleus, Cytoplasm, DNA (Cytosine-5-)-Methyltransferase, DNA Methylation, Mammalian Embryo, Embryonic Development, Genetic Epigenesis, Green Fluorescent Proteins, Isoenzymes, Protein Transport, Recombinant Fusion Proteins, Animals, Mice
Source:BMC Developmental Biology
Publisher:BioMed Central
Page Range:18
Date:24 August 2005
Official Publication:https://doi.org/10.1186/1471-213X-5-18
PubMed:View item in PubMed

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