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Glycodelin A and differentiation of first trimester trophoblast cells in vitro

Item Type:Article
Title:Glycodelin A and differentiation of first trimester trophoblast cells in vitro
Creators Name:Jeschke, U. and Richter, D.U. and Reimer, T. and Bergemann, C. and Briese, V. and Karsten, U. and Mylonas, I. and Kupka, M.S. and Wiest, I. and Friese, K.
Abstract:Aim: The glycoprotein, glycodelin A (GdA) is a main product of the maternal decidua in the first trimester of pregnancy and is secreted into the amniotic fluid. The purpose of this study was to investigate the effect of GdA on secretion and surface markers of isolated first trimester trophoblasts in vitro. Methods: Cytotrophoblasts were prepared from human first trimester placentae and incubated with varying concentrations of GdA or transfected separately with the expression plasmid of GdA. Supernatants were assayed for human chorionic gonadotropin (hCG) protein concentrations. Expression of human placental lactogen (hPL), mucin 1 (MUC1) and the Thomsen-Friedenreich (TF) epitope was analysed in stimulated trophoblast cells and in unstimulated controls by immunocytochemistry. Results: Glycodelin A induced a reduced expression of hPL compared with unstimulated controls. Expression of MUC1 was not affected by GdA. Freshly isolated trophoblast cells showed no TF expression but became positive for this antigen after 96 h of cultivation. GdA-stimulated trophoblast cells inhibited TF expression after 96 h of cultivation. GdA plasmids induced a significantly higher hCG production in transfected cells than in cells transfected with the empty plasmid. Conclusions: The results obtained in this study suggest that GdA is involved in the differentiation of trophoblast cells. The treatment of GdA plasmid transfected trophoblast cells stimulated hCG production in isolated trophoblast cells and inhibited hPL and TF expression, suggesting a functional link between hCG and GdA.
Keywords:Cell Culture, First Trimenon, Glycodelin A, Trophoblast
Source:Archives of Gynecology and Obstetrics
Page Range:151-159
Date:1 August 2005
Official Publication:https://doi.org/10.1007/s00404-004-0682-2
PubMed:View item in PubMed

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