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Mouse knockout models of hypertension

Item Type:Book Section
Title:Mouse knockout models of hypertension
Creators Name:Bader, M.
Abstract:Gene-targeting technology allows the planned alteration of any gene in the mouse genome and has been very successfully employed to study the function of numerous gene products in a complete animal. The method includes the design of a suitable targeting construct, its transfection into pluripotential embryonic stem cells, selection for cells in which one allele of the endogenous gene has been exchanged for the construct by homologous recombination, and the transfer of these cells into host blastocysts. The blastocysts are then transferred into the uterus of a pseudopregnant foster mother giving birth to chimeras, which are bred to yield heterozygous and finally homozygous mutant mice for the targeted gene.
Keywords:Embryonic stem cells, Homologous recombination, Gene targeting, Knockout mouse, Electroporation, Double selection, Gancyclovir, Functional Genomics, Chimera, Pseudopregnancy, Animals, Mice
Series Name:Methods in Molecular Medicine ; 108
Title of Book:Hypertension: Methods and Protocols
Publisher:Humana Press
Page Range:17-32
Date:25 October 2004
Official Publication:https://doi.org/10.1385/1-59259-850-1:017
PubMed:View item in PubMed

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