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Production of transgenic models in hypertension

Item Type:Book Section
Title:Production of transgenic models in hypertension
Creators Name:Popova, E. and Bader, M. and Krivokharchenko, A.
Abstract:This chapter describes the generation of transgenic mice and rats by microinjection of DNA constructs into the pronucleus of a zygote. The transgene DNA is randomly integrated as several tandem copies at one site into the genome and is transmitted to the offspring of the founder animal derived from the injected embryo, thereby creating a stable transgenic line. The technology includes the following steps: design and generation of the transgene construct, superovulation of donor animals, isolation of fertilized oocytes, microinjection of the transgene construct into one pronucleus of the zygotes, transfer of injected embryos into the oviduct of a foster mother, and identification of transgenic animals in the offspring.
Keywords:Transgenic rat, Transgenic mouse, Microinjection, Superovulation, Oviduct transfer, Promoter, Embryo culture, Zygote, Pronucleus, Vasectomy, Pseudopregnancy, Genotyping
Series Name:Methods in Molecular Medicine ; 108
Title of Book:Hypertension: Methods and Protocols
Publisher:Humana Press
Page Range:33-50
Date:25 October 2004
Official Publication:https://doi.org/10.1385/1-59259-850-1:033
PubMed:View item in PubMed

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