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Parkin interacts with the proteasome subunit alpha4

Item Type:Article
Title:Parkin interacts with the proteasome subunit alpha4
Creators Name:Daechsel, J.C. and Luecking, C.B. and Deeg, S. and Schultz, E. and Lalowski, M. and Casademunt, E. and Corti, O. and Hampe, C. and Patenge, N. and Vaupel, K. and Yamamoto, A. and Dichgans, M. and Brice, A. and Wanker, E.E. and Kahle, P.J. and Gasser, T.
Abstract:Mutations in the parkin gene encoding an E3 ligase are responsible for autosomal recessive Parkinson's disease. Putative parkin substrates and interacting partners have been identified, but the molecular mechanism underlying parkin-related neurodegeneration is still unclear. We have identified the 20S proteasomal subunit α4 (synonyms: PSMA7, XAPC7, subunit alpha type 7) as a new interacting partner of parkin. The C-terminal IBR-RING domain of parkin and the C-terminal part of α4 were essential for the interaction. Biochemical studies revealed that α4 was not a substrate for parkin-dependent ubiquitylation. Putative functions of the interaction might therefore be substrate presentation to the proteasome or regulation of proteasomal activity. Full-length parkin and parkin lacking the N-terminal ubiquitin-like domain slightly increased the proteasomal activity in HEK 293T cells, in line with the latter hypothesis.
Keywords:Interaction, Parkin, Proteasomal activity, Proteasome subunit {alpha}4, PSMA7, Animals, Rats
Source:FEBS Letters
ISSN:0014-5793
Publisher:Elsevier (The Netherlands)
Volume:579
Number:18
Page Range:3913-3919
Date:18 July 2005
Official Publication:https://doi.org/10.1016/j.febslet.2005.06.003
PubMed:View item in PubMed

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