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| Item Type: | Article |
|---|---|
| Title: | The MEK1 scaffolding protein MP1 regulates cell spreading by integrating PAK1 and Rho signals |
| Creators Name: | Pullikuth, A., McKinnon, E., Schaeffer, H.J. and Catling, A.D. |
| Abstract: | How the extracellular signal-regulated kinase (ERK) cascade regulates diverse cellular functions, including cell proliferation, survival, and motility, in a context-dependent manner remains poorly understood. Compelling evidence indicates that scaffolding molecules function in yeast to channel specific signals through common components to appropriate targets. Although a number of putative ERK scaffolding proteins have been identified in mammalian systems, none has been linked to a specific biological response. Here we show that the putative scaffold protein MEK partner 1 (MP1) and its partner p14 regulate PAK1-dependent ERK activation during adhesion and cell spreading but are not required for ERK activation by platelet-derived growth factor. MP1 associates with active but not inactive PAK1 and controls PAK1 phosphorylation of MEK1. Our data further show that MP1, p14, and MEK1 serve to inhibit Rho/Rho kinase functions necessary for the turnover of adhesion structures and cell spreading and reveal a signal-channeling function for a MEK1/ERK scaffold in orchestrating cytoskeletal rearrangements important for cell motility. Copyright |
| Keywords: | Actin Depolymerizing Factors, Amino Acid Sequence, Cell Adhesion, Cell Line, Enzyme Activation, Extracellular Signal-Regulated MAP Kinases, Focal Adhesions, Intracellular Signaling Peptides and Proteins, MAP Kinase Kinase 1, Microfilament Proteins, Molecular Sequence Data, Protein Binding, Protein-Serine-Threonine Kinases, Proteins, Rho GTP-Binding Proteins, Sequence Alignment, Signal Transduction, Signal Transducing Adaptor Proteins, Small Interfering RNA, Two-Hybrid System Techniques, Animals, Rats |
| Source: | Molecular and Cellular Biology |
| ISSN: | 0270-7306 |
| Publisher: | American Society for Microbiology |
| Volume: | 25 |
| Number: | 12 |
| Page Range: | 5119-5133 |
| Date: | 1 June 2005 |
| Official Publication: | https://doi.org/10.1128/MCB.25.12.5119-5133.2005 |
| PubMed: | View item in PubMed |
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