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Recruitment of DNA methyltransferase I to DNA repair sites

Official URL:http://dx.doi.org/10.1073/pnas.0501034102
PubMed:View item in PubMed
Creators Name:Mortusewicz, O. and Schermelleh, L. and Walter, J. and Cardoso, M.C. and Leonhardt, H.
Journal Title:Proceedings of the National Academy of Sciences of the United States of America
Journal Abbreviation:Proc Natl Acad Sci U S A
Page Range:8905-8909
Date:21 June 2005
Keywords:DNA Methylation, Dnmt1, Microirradiation, Proliferating Cell Nuclear Antigen, Animals, Mice
Abstract:In mammalian cells, the replication of genetic and epigenetic information is directly coupled; however, little is known about the maintenance of epigenetic information in DNA repair. Using a laser microirradiation system to introduce DNA lesions at defined subnuclear sites, we tested whether the major DNA methyltransferase (Dnmt1) or one of the two de novo methyltransferases (Dnmt3a, Dnmt3b) are recruited to sites of DNA repair in vivo. Time lapse microscopy of microirradiated mammalian cells expressing GFP-tagged Dnmt1, Dnmt3a, or Dnmt3b1 together with red fluorescent protein-tagged proliferating cell nuclear antigen (PCNA) revealed that Dnmt1 and PCNA accumulate at DNA damage sites as early as 1 min after irradiation in S and non-S phase cells, whereas recruitment of Dnmt3a and Dnmt3b was not observed. Deletion analysis showed that Dnmt1 recruitment was mediated by the PCNA-binding domain. These data point to a direct role of Dnmt1 in the restoration of epigenetic information during DNA repair.
Publisher:National Academy of Sciences (U.S.A.)
Additional Information:Copyright (c) 2005 by The National Academy of Sciences
Item Type:Article

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