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Neurotractin/kilon promotes neurite outgrowth and is expressed on reactive astrocytes after entorhinal cortex lesion

Item Type:Article
Title:Neurotractin/kilon promotes neurite outgrowth and is expressed on reactive astrocytes after entorhinal cortex lesion
Creators Name:Schaefer, M. and Braeuer, A.U. and Savaskan, N.E. and Rathjen, F.G. and Bruemmendorf, T.
Abstract:The IgLON subgroup of the immunoglobulin superfamily consists of four members that are thought to be important in neural cell-cell recognition. Here, we cloned and characterized the murine IgLON subgroup member neurotractin/kilon, in the context of brain development and axonal regeneration. Neurotractin/kilon was found to be upregulated during brain development and is expressed on neurites of primary hippocampal neurons. To elucidate a potential role for neurotractin/kilon during regeneration in the CNS, we performed lesions in the entorhinal cortex, and showed that the expression of neurotractin/kilon is induced on reactive astrocytes. Notably, the expression on reactive astrocytes appears specifically in the denervated outer molecular layer of the dentate gyrus, where regenerative axon sprouting occurs. In vitro assays demonstrated that neurotractin/kilon attracts hippocampal axons in the stripe assay and that astroglial neurotractin/kilon promotes neurite outgrowth. These results suggest a function for neurotractin/kilon as a trans-neural growth-promoting factor for outgrowing axons following hippocampal denervation
Keywords:Amino Acid Sequence Homology, Animal Disease Models, Astrocytes, Avian Proteins, Brain Injuries, Cercopithecus Aethiops, Chick Embryo, Complementary DNA, COS Cells, Denervation, Dentate Gyrus, Entorhinal Cortex, Gliosis, Growth Substances, Inbred C57BL Mice, Membrane Proteins, Molecular Sequence Data, Nerve Regeneration, Nerve Tissue Proteins, Neuronal Cell Adhesion Molecules, Neuronal Plasticity, Nucleic Acid Sequence Homology, Animals, Mice, Rats
Source:Molecular and Cellular Neuroscience
Publisher:Academic Press
Page Range:580-590
Date:1 January 2005
Official Publication:https://doi.org/10.1016/j.mcn.2005.04.010
PubMed:View item in PubMed

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