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Stimulation of hCG protein and mRNA in first trimester villous cytotrophoblast cells in vitro by glycodelin A

Item Type:Article
Title:Stimulation of hCG protein and mRNA in first trimester villous cytotrophoblast cells in vitro by glycodelin A
Creators Name:Jeschke, U. and Karsten, U. and Reimer, T. and Richter, D.U. and Bergemann, C. and Briese, V. and Mylonas, L. and Friese, K.
Abstract:Aim: Human chorionic gonadotropin (hCG) is produced by fetal trophoblast cells and secreted into maternal circulation mainly in the first trimester of pregnancy. Another glycoprotein, glycodelin A, is one of the main products of the maternal decidua during this period. The purpose of this study was to investigate the effect of glycodelin A on hCG release by isolated cytotrophoblast cells in vitro. Methods: Cytotrophoblast cells were prepared from human first trimester placenta and incubated with varying concentrations of glycodelin A. Supernatants were assayed for hCG protein concentrations, and quantification of beta hCG mRNA was carried out by RT-PCR. Expression of hCG was analysed in stimulated trophoblast cells and in unstimulated controls by immunocytochemistry. Results: Glycodelin A induces a dose-dependent increase of hCG production. An increase of hCG expression was measured at 100 and 200 mg/mL glycodelin-A treatment in trophoblast cell culture by TaqMan assay on mRNA level. We found a moderate staining of hCG in control trophoblast cells, whereas a strong hCG staining was seen in glycodelin A-treated trophoblast cells. Conclusions: HCG is a marker for the differentiation process of trophoblast cells. Our results suggest that glycodelin A secreted by the decidualized endometrium is involved in the regulation of hormones produced by the trophoblast.
Keywords:First Trimester Villous Cytotrophoblast Cell Culture, Glycodelin A, Regulation of HCG
Source:Journal of Perinatal Medicine
Publisher:Walter de Gruyter & co
Page Range:212-218
Date:1 January 2005
Official Publication:https://doi.org/10.1515/JPM.2005.039
PubMed:View item in PubMed

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