Distinction of acute lymphoblastic leukemia from acute myeloid leukemia through microarray-based DNA methylation analysis

Item Type:	Article
Title:	Distinction of acute lymphoblastic leukemia from acute myeloid leukemia through microarray-based DNA methylation analysis
Creators Name:	Scholz, C. and Nimmrich, I. and Burger, M. and Becker, E. and Doerken, B. and Ludwig, W.D. and Maier, S.
Abstract:	Patterns of DNA methylation are substantially altered in malignancies compared to normal tissue, with both genome-wide hypomethylation and regional increase of cytosine methylation at dinucleotides of cytosine and guanine, i.e., CpG dinucleotides. While genome-wide hypomethylation renders chromosomes instable, hypermethylation of CpGs in promoter regions is generally associated with transcriptional silencing, e.g., of tumor suppressor genes. To investigate whether disease-specific methylation profiles exist for different entities of acute leukemia, a microarray-based DNA methylation analysis simultaneously assessing 249 CpG dinucleotides originating from 57 genes was employed. Hereby, samples from precursor B-cell acute lymphoblastic leukemia (ALL) could be distinguished from cases of acute myeloid leukemia by virtue of N33, EGR4, CDC2, CCND2, or MOS hypermethylation in ALL.
Keywords:	Acute leukemia, Methylation, Microarray
Source:	Annals of Hematology
ISSN:	0939-5555
Publisher:	Springer
Volume:	84
Page Range:	236-244
Date:	1 January 2005
Official Publication:	https://doi.org/10.1007/s00277-004-0969-1
PubMed:	View item in PubMed

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