Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Necrotic death but not irradiation abolishes costimulation of T-cell effector functions and survival by CD80-expressing tumor cells

Item Type:Article
Title:Necrotic death but not irradiation abolishes costimulation of T-cell effector functions and survival by CD80-expressing tumor cells
Creators Name:Staerck, L. and Scholz, C. and Blankenstein, T. and Doerken, B. and Daniel, P.T.
Abstract:Tumor vaccination by the use of gene-modified cancer cells that provide costimulatory signals has been successfully applied in pre-clinical animal models and is currently evaluated in a variety of clinical settings. In previous work, we demonstrated the efficacy of B7.1/CD80 to promote tumor immunity in syngeneic murine models and to prevent deletion of activated T cells by activation-induced cell death (AICD). In clinical trials, tumor cell vaccines are generally inactivated to avoid transfer of five tumor cells, i.e., additional tumor burden. Previous data indicated, however, that inactivation of tumor cells by lethal ionizing irradiation abrogates tumor vaccination by CD80-expressing cells. Here, we compare living and irradiated allogeneic tumor cells regarding their capacity to induce T-cell effector functions and their propensity to interfere with T-cell deletion by apoptosis. Both lethally irradiated and nonirradiated tumor cells facilitated T-cell proliferation, tumor cell lysis, and interfered with T-cell AICD to a similar extent. In contrast, necrotic tumor cells failed to costimulate T-cell effector functions. Thus, irradiation does not seem to hamper tumor cell-mediated costimulation of T-cell effector functions. In contrast, necrosis of gene-modified tumor cells abrogates costimulation of T cells by CD80-expressing cells.
Keywords:Apoptosis, CD80, Costimulation, Irradiation, Necrosis
Source:International Journal of Cancer
ISSN:0020-7136
Publisher:Wiley (U.S.A.)
Volume:116
Number:1
Page Range:78-86
Date:1 January 2005
Official Publication:https://doi.org/10.1002/ijc.20792
PubMed:View item in PubMed

Repository Staff Only: item control page

Open Access
MDC Library