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E2F transcription factors and pRb pocket proteins in cell cycle progression

Item Type:Article
Title:E2F transcription factors and pRb pocket proteins in cell cycle progression
Creators Name:Hauck, L. and von Harsdorf, R.
Abstract:The E2F-family of transcripion factors exerts fascinating and contrasting functions in transcriptional repression and activation of genes regulating proliferation, apoptosis, and differentiation. E2F is principally regulated by its temporal association with retinoblastoma pocket protein (pRb) family members. In turn, pRb is regulated through phosphorylation by cyclin-dependent kinase (cdk). The activity of cdk is negatively regulated by cdk-inhibitors, exemplified by p16INK4a, p21CIP1, and p27KIP1. Therefore, positive and negative signaling events converge on E2F activity resulting in distinct growth-controling and apoptotic activities. Here we describe the immunocytochemical detection of E2F, genomic DNA, BrdU-incorporation, and mitosis in cardiomyoctes. A detailed protocol is given to illustrate this technique in primary heart muscle cells
Keywords:Bromodeoxyuridine, Cardiac Myocytes, Cell Cycle, Cell Cycle Proteins, Cell Separation, Cultured Cells, DNA-Binding Proteins, E2F Transcription Factors, Immobilized Cells, Immunohistochemistry, Indirect Fluorescent Antibody Technique, Newborn Animals, Retinoblastoma Protein, Transcription Factors, Animals, Rats
Source:Methods in Molecular Biology
ISSN:1064-3745
Publisher:Springer / Humana Press (U.S.A.)
Volume:296
Page Range:239-245
Date:1 January 2005
PubMed:View item in PubMed

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