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| Item Type: | Article |
|---|---|
| Title: | Botulinum neurotoxin C initiates two different programs for neurite degeneration and neuronal apoptosis |
| Creators Name: | Berliocchi, L. and Fava, E. and Leist, M. and Horvat, V. and Dinsdale, D. and Read, D. and Nicotera, P. |
| Abstract: | Clostridial neurotoxins are bacterial endopeptidases that cleave the major SNARE proteins in peripheral motorneurons. Here, we show that disruption of synaptic architecture by botulinum neurotoxin C1 (BoNT/C) in central nervous system neurons activates distinct neurodegenerative programs in the axo-dendritic network and in the cell bodies. Neurites degenerate at an early stage by an active caspase-independent fragmentation characterized by segregation of energy competent mitochondria. Later, the cell body mitochondria release cytochrome c, which is followed by caspase activation, apoptotic nuclear condensation, loss of membrane potential, and, finally, cell swelling and lysis. Recognition and scavenging of dying processes by glia also precede the removal of apoptotic cell bodies, in line with a temporal and spatial segregation of different degenerative processes. Our results suggest that, in response to widespread synaptic damage, neurons first dismantle their connections and finally undergo apoptosis, when their spatial relationships are lost. |
| Keywords: | Apoptosis, Botulinum Toxins, Cultured Cells, Cerebellum, Cytochromes c, Cytoskeleton, Electron Scanning Microscopy, Mitochondria, Nerve Tissue Proteins, Neurites, Neuroglia, Neurons, Neurotransmitter Agents, Synapses, Animals, Mice |
| Source: | Journal of Cell Biology |
| ISSN: | 0021-9525 |
| Publisher: | Rockefeller University Press |
| Volume: | 168 |
| Number: | 4 |
| Page Range: | 607-618 |
| Date: | 14 February 2005 |
| Additional Information: | Copyright (c) 2005 by Rockefeller University Press |
| Official Publication: | https://doi.org/10.1083/jcb.200406126 |
| PubMed: | View item in PubMed |
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