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Dynamic complex formation during the yeast cell cycle

Official URL:https://doi.org/10.1126/science.1105103
PubMed:View item in PubMed
Creators Name:de Lichtenberg, U. and Jensen, L.J. and Brunak, S. and Bork, P.
Journal Title:Science
Journal Abbreviation:Science
Volume:307
Number:5710
Page Range:724-727
Date:4 February 2005
Keywords:S Cerevisiae CDC28 Protein Kinase, Cell Cycle, Cell Cycle Proteins, Cyclins, Cytoskeleton, DNA Repair, DNA Replication, Fungal Gene Expression Regulation, Fungal Genes, Mitosis, Oligonucleotide Array Sequence Analysis, Phosphorylation, Protein Interaction Mapping, Protein Subunits, Saccharomyces Cerevisiae, Genetic Transcription
Abstract:To analyze the dynamics of protein complexes during the yeast cell cycle, we integrated data on protein interactions and gene expression. The resulting time-dependent interaction network places both periodically and constitutively expressed proteins in a temporal cell cycle context, thereby revealing previously unknown components and modules. We discovered that most complexes consist of both periodically and constitutively expressed subunits, which suggests that the former control complex activity by a mechanism of just-in-time assembly. Consistent with this, we show that additional regulation through targeted degradation and phosphorylation by Cdc28p (Cdk1) specifically affects the periodically expressed proteins.
ISSN:0036-8075
Publisher:American Association for the Advancement of Science (U.S.A.)
Item Type:Article

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