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New modules for the repeated internal and N-terminal epitope tagging of genes in Saccharomyces cerevisiae

Item Type:Article
Title:New modules for the repeated internal and N-terminal epitope tagging of genes in Saccharomyces cerevisiae
Creators Name:Gauss, R. and Trautwein, M. and Sommer, T. and Spang, A.
Abstract:Epitope tagging is a powerful method for the rapid analysis of protein function. In Saccharomyces cerevisiae epitope tags are introduced easily into chromosomal loci by homologous recombination using a simple PCR-based strategy. Although quite a number of tools exist for C-terminal tagging as well as N-terminal tagging of proteins expressed by heterologous promoters, there are only very limited possibilities to tag proteins at the N-terminus and retain the endogenous expression level. Furthermore, no PCR-templates for internal tagging have been reported. Here we describe new modules that are suitable for both the repeated N-terminal and internal tagging of proteins, leaving their endogenous promoters intact. The tags include 6xHA, 9xMyc, yEGFP, TEV-GST-6xHIS, ProtA, TEV-ProtA and TEV-ProtA-7xHIS in conjunction with different heterologous selection markers.
Keywords:Epitope Tagging, Saccharomyces Cerevisiae, Yeast
Source:Yeast
ISSN:0749-503X
Publisher:John Wiley & Sons Ltd (England)
Volume:22
Number:1
Page Range:1-12
Date:1 January 2005
Official Publication:https://doi.org/10.1002/yea.1187
PubMed:View item in PubMed

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