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Lipid-mediated gene transfer of acidic fibroblast growth factor into human corneal endothelial cells

Item Type:Article
Title:Lipid-mediated gene transfer of acidic fibroblast growth factor into human corneal endothelial cells
Creators Name:Dannowski, H. and Bednarz, J. and Reszka, R. and Engelmann, K. and Pleyer, U.
Abstract:The aim of this study was to optimize non-viral gene transfer conditions and investigate the effect of fibroblast growth factor-1 (FGF-1) gene transfer on human corneal endothelial cell (HCEC) proliferation. Five non-viral vectors (Lipofectin™, DMRIE-C™, DAC-30, Effectene™, FuGene™6) were used to transfect HCEC with plasmids coding for enhanced green fluorescent protein (EGFP) and FGF-1. Transfection efficiency and toxicity (n=6) were quantified and optimized using the EGFP construct by FACS-analysis. Using optimal conditions HCEC were transfected with the FGF-1 plasmid and cell proliferation as well as expression of FGF-1 were determined at days 4 and 7 by counting and western blotting, respectively. Lipofectin (17±2·02%) transfected HCEC more successfully than DMRIE-C (11±1·46%), Effectene (9±0·62%), FuGene (9±0·93%) and DAC-30 (7±0·59%). Toxicity of the lipids ranged from 2 to 4%. Optimal HCEC proliferation was achieved with DAC-30/FGF-1 (P<0·05), whereas all other vectors did not result in significantly increased cell proliferation. However, all of the transfected cells produced FGF-1 in different amounts as indicated by western blotting. Efficient and almost non-toxic transfer of the FGF-1 gene into HCEC can be successfully achieved by lipid-based techniques. Using optimal conditions significantly increased cell proliferation was independent on gene transfer efficiency. This may indicate that even a low transfection rate is sufficient to produce a concentration of FGF-1 that will have a stimulatory effect on HCECs.
Keywords:Cell Proliferation, Corneal Endothelial Cells, Fibroblast Growth Factor, Gene Transfer, Liposomes
Source:Experimental Eye Research
Publisher:Ademic Press Ltd Elsevier Science Ltd
Page Range:93-101
Date:1 January 2005
Official Publication:https://doi.org/10.1016/j.exer.2004.08.024
PubMed:View item in PubMed

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