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Effect of Add1 gene transfer on blood pressure in reciprocal congenic strains of Milan rats

Item Type:Article
Title:Effect of Add1 gene transfer on blood pressure in reciprocal congenic strains of Milan rats
Creators Name:Tripodi, G. and Florio, M. and Ferrandi, M. and Modica, R. and Zimdahl, H. and Huebner, N. and Ferrari, P. and Bianchi, G.
Abstract:Genetic variants of α adducin (ADD1) taken alone or in interaction with those of β (ADD2) and β (ADD3) subunits have been associated with primary hypertension in humans and in Milan hypertensive (MHS) rats. In this study, we report the dissection of the individual contribution of each rat Add gene to blood pressure, by congenic substitution mapping. Congenic strains were developed by introgressing Add1, Add2, and Add3 genes (and chr14, chr4, and chr1 associated segments) of MHS in the Milan normotensive rat (MNS) genetic background (MNS.H-Add1, MNS.H-Add2, and MNS.H-Add3) and vice versa (MHS.N-Add1, MHS.N-Add2, and MHS.N-Add3). Systolic blood pressure (SBP) of MNS.H-Add1 rats was significantly higher (+10 mmHg) than that of MNS, whereas SBP of MHS.N-Add1 was significantly lower (-10 mmHg) than that of MHS. The differences account for 43% of the blood pressure differences between MHS and MNS. In contrast, SBPs of Add2 and Add3 congenic strains were not different from those of the correspondent recipient parental strain. The fine mapping of chr14 congenic segment supports the identity of blood pressure QTL with Add1 gene.
Keywords:Adducin, Congenic Strains, Genetics, Hypertension, Inbred MHS, Animals, Rats
Source:Biochemical and Biophysical Research Communications
ISSN:0006-291X
Publisher:Academic Press (U.S.A.)
Volume:324
Number:2
Page Range:562-568
Date:1 January 2004
Official Publication:https://doi.org/10.1016/j.bbrc.2004.09.079
PubMed:View item in PubMed

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