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Essential requirement of CCAAT/enhancer binding proteins in embryogenesis

Item Type:Article
Title:Essential requirement of CCAAT/enhancer binding proteins in embryogenesis
Creators Name:Begay, V. and Smink, J. and Leutz, A.
Abstract:The CCAAT/enhancer binding proteins C/EBPα and C/EBPβ are related transcription factors that are important for the function of various organs in the postnatal mouse. Gene replacement and tissue culture experiments have suggested partial redundancy of both transcription factors. Here we show that mouse embryos deficient of both C/EBPα and C/EBPβ (C/EBPαβ-/-) die between embryonic day 10 (E10) and E11 and display defective placentas. In situ hybridization revealed that C/EBPα and C/EBPβ are coexpressed in the chorionic plate at E9.5 and later in the trophoblasts of the labyrinthine layer. In C/EBPαβ -/- placentas, allantoic blood vessels invaded the chorion; however, vessel expansion and development of the labyrinthine layer was impaired. Furthermore, a single copy of either C/EBPα in the absence of C/EBPβ or C/EBPβ in the absence of C/EBPα is sufficient to complete development, suggesting complementation of these C/EBPs during embryogenesis. A single copy of C/EBPα in the absence of C/EBPβ, however, fails to rescue survival after birth, suggesting naploinsufficiency of C/EBPα in newborns. Our data thus reveal novel essential, redundant, and dosage dependent functions of C/EBPs.
Keywords:Base Sequence, CCAAT-Enhancer-Binding Protein-Apha, CCAAT-Enhancer-Binding Protein-Beta, DNA, Fetal Death, Fetal Development, Gene Expression, Genetic Markers, In Situ Hybridization, Knockout Mice, Placenta, Pregnancy, Animals, Mice
Source:Molecular and Cellular Biology
ISSN:0270-7306
Publisher:American Society for Microbiology
Volume:24
Number:22
Page Range:9744-9751
Date:1 November 2004
Official Publication:https://doi.org/10.1128/MCB.24.22.9744-9751.2004
PubMed:View item in PubMed

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