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Role of CCL21 in recruitment of T precursor cells to fetal thymus

Official URL:https://doi.org/10.1182/blood-2004-04-1369
PubMed:View item in PubMed
Creators Name:Liu, C. and Ueno, T. and Kuse, S. and Saito, F. and Nitta, T. and Piali, L. and Nakano, H. and Kakiuchi, T. and Lipp, M. and Hollander, G.A. and Takahama, Y.
Journal Title:Blood
Journal Abbreviation:Blood
Volume:105
Number:1
Page Range:31-39
Date:1 January 2005
Keywords:Antibodies, CC Chemokines, Chemokine CCL21, Chemokine CXCL12, Cultured Cells, Fetal Blood, Fetus, Histocompatibility Antigens Class II, Inbred C57BL Mice, Knockout Mice, Leukocyte Chemotaxis, Liver, Messenger RNA, Stem Cells, T-Lymphocytes, Thymus Gland, Time Factors, Animals, Mice
Abstract:During embryonic development, T-lymphoid precursor cells colonize the thymus. Chemoattraction by the fetal thymus is thought to mediate T-precursor cell colonization. However, the molecules that attract T-precursor cells to the thymus remain unclear. By devising time-lapse visualization in culture, the present results show that alymphoid fetal thymus lobes attract T-precursor cells from fetal liver or fetal blood. CD4–CD8–CD25–CD44+ fetal thymocytes retained the activity to specifically re-enter the thymus. The attraction was predominantly due to I-A–expressing thymic epithelial cells and was mediated by pertussis toxin-sensitive G-protein signals. Among the chemokines produced by the fetal thymus, CCL21, CCL25, and CXCL12 could attract CD4–CD8–CD25–CD44+ fetal thymocytes. However, fetal thymus colonization was markedly diminished by neutralizing antibodies specific for CCL21 and CCL25, but not affected by anti-CXCL12 antibody. Fetal thymus colonization was partially defective in CCL21-deficient plt/plt mice and was further diminished by anti-CCL25 antibody. These results indicate that CCL21 is involved in the recruitment of T-cell precursors to the fetal thymus and suggest that the combination of CCL21 and CCL25 plays a major role in fetal thymus colonization.
ISSN:0006-4971
Publisher:American Society of Hematology (U.S.A.)
Item Type:Article

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