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Role of T-type calcium current in identified D-hair mechanoreceptor neurons studied in vitro

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Item Type:Article
Title:Role of T-type calcium current in identified D-hair mechanoreceptor neurons studied in vitro
Creators Name:Dubreuil, A.S. and Boukhaddaoui, H. and Desmadryl, G. and Martinez-Salgado, C. and Moshourab, R. and Lewin, G.R. and Carroll, P. and Valmier, J. and Scamps, F.
Abstract:Different subsets of dorsal root ganglion (DRG) mechanoreceptors transduce low- and high-intensity mechanical stimuli. It was shown recently that, in vivo, neurotrophin-4 (NT-4)-dependent D-hair mechanoreceptors specifically express a voltage-activated T-type calcium channel (Ca(v)3.2) that may be required for their mechanoreceptive function. Here we show that D-hair mechanoreceptors can be identified in vitro by a rosette-like morphology in the presence of NT-4 and that these rosette neurons are almost all absent in DRG cultures taken from NT-4 knock-out mice. In vitro identification of the D-hair mechanoreceptor allowed us to explore the electrophysiological properties of these cells. We demonstrate that the T-type Ca(v)3.2 channel induced slow membrane depolarization that contributes to lower the voltage threshold for action potential generation and controls spike latency after stimulation of D-hair mechanoreceptors. Indeed, the properties of the T-type amplifier are particularly well suited to explain the high sensitivity of D-hair mechanoreceptors to slowly moving stimuli.
Keywords:T-type Calcium Channel, Cav3.2, Neurotrophin, Mechanotransduction, D-Hair Neuron, Dorsal Root Ganglion, Animals, Mice
Source:Journal of Neuroscience
ISSN:0270-6474
Publisher:Society for Neuroscience (U.S.A.)
Volume:24
Number:39
Page Range:8480-8484
Date:29 September 2004
Additional Information:Copyright (c) 2004 by The Society for Neuroscience
Official Publication:https://doi.org/10.1523/JNEUROSCI.1598-04.2004
PubMed:View item in PubMed

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