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Low-volume jet injection for efficient nonviral in vivo gene transfer

Item Type:Review
Title:Low-volume jet injection for efficient nonviral in vivo gene transfer
Creators Name:Walther, W. and Stein, U. and Fichtner, I. and Schlag, P.M.
Abstract:The transfer of naked deoxyribonucleic acid (DNA) represents an alternative to viral and liposomal gene transfer technologies for gene therapy applications. Various procedures are employed to deliver naked DNA into the desired cells or tissues in vitro and in vivo, such as by simple needle injection, particle bombardment, in vivo electroporation or jet injection. Among the various nonviral gene delivery technologies jet injection is gaining increasing acceptance because it allows gene transfer into different tissues with deeper penetration of the applied naked DNA. The versatile hand-held Swiss jet injector uses pressurized air to force small volumes of 3 to 10 {my}L of naked DNA into targeted tissues. The {beta}-galactosidase (LacZ) reporter gene construct and tumor necrosis factor {alpha} gene-expressing vectors were successfully jet injected at a pressure of 3.0 bar into xenotransplanted human tumor models of colon carcinoma. Qualitative and quantitative expression analysis of jet injected tumor tissues revealed the efficient expression of these genes in the tumors. Using this Swiss jet-injector prototype repeated jet injections of low volumes (3–10 {my}L) into one target tissue can easily be performed. The key parameters of in vivo jet injection such as jet injection volume, pressure, jet penetration into the tumor tissue, DNA stability have been defined for optimized nonviral gene therapy. These studies demonstrate the applicability of the jet injection technology for the efficient and simultaneous in vivo gene transfer of two different plasmid DNAs into tumors. It can be employed for nonviral gene therapy of cancer using minimal amounts of naked DNA.
Keywords:Nonviral Gene Transfer, Jet-Injection, Naked DNA
Source:Molecular Biotechnology
ISSN:1073-6085
Publisher:Humana Press Inc (U.S.A.)
Volume:28
Number:2
Page Range:121-128
Date:1 January 2004
Official Publication:https://doi.org/10.1385/MB:28:2:121
PubMed:View item in PubMed

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