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Rat corin gene: molecular cloning and reduced expression in experimental heart failure

Item Type:Article
Title:Rat corin gene: molecular cloning and reduced expression in experimental heart failure
Creators Name:Langenickel, T.H. and Pagel, I. and Buttgereit, J. and Tenner, K. and Lindner, M. and Dietz, R. and Willenbrock, R. and Bader, M.
Abstract:Stored cardiac pro-atrial natriuretic peptide (pro-ANP) is converted to ANP and released upon stretch from the atria into the circulation. Corin is a serin protease with pro-ANP-converting properties and may be the rate-limiting enzyme in ANP release. This study was aimed to clone and sequence corin in the rat and to analyze corin mRNA expression in heart failure when ANP release upon stretch is blunted. Full-length cDNA of rat corin was obtained from atrial RNA by RT-PCR and sequenced. Tissue distribution as well as regulation of corin mRNA expression in the atria were determined by RT-PCR and RNase protection assay. Heart failure was induced by an infrarenal aortocaval shunt. Stretch was applied to the left atrium in a working heart modus, and ANP was measured in the perfusates. The sequence of rat corin cDNA was found to be 93.6% homologous to mouse corin cDNA. Corin mRNA was expressed almost exclusively in the heart with highest concentrations in both atria. The aortocaval shunt led to cardiac hypertrophy and heart failure. Stretch-induced ANP release was blunted in shunt animals (control 1,195 ± 197 fmol·min-1·g -1; shunt: 639 ± 99 fmol·min-1·g -1, P < 0.05). Corin mRNA expression was decreased in both atria in shunt animals [right atrium: control 0.638 ± 0.004 arbitrary units (AU), shunt 0.566 ± 0.014 AU, P < 0.001; left atrium: control 0.564 ± 0.009 AU, shunt 0.464 ± 0.009 AU, P < 0.001]. Downregulation of atrial corin mRNA expression may be a novel mechanism for the blunted ANP release in heart failure.
Keywords:Atrial Natiuretic Factor, Gene Expression, Animals, Rats
Source:American Journal of Physiology Heart and Circulatory Physiology
ISSN:0363-6135
Publisher:American Physiological Society (U.S.A.)
Volume:287
Number:4
Page Range:H1516-H1521
Date:1 January 2004
Official Publication:https://doi.org/10.1152/ajpheart.00947.2003
PubMed:View item in PubMed

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