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Detection of a novel sense-antisense RNA-hybrid structure by RACE experiments on endogenous troponin I antisense RNA

Item Type:Article
Title:Detection of a novel sense-antisense RNA-hybrid structure by RACE experiments on endogenous troponin I antisense RNA
Creators Name:Bartsch, H. and Voigtsberger, S. and Baumann, G. and Morano, I. and Luther, H.P.
Abstract:Conformational changes in the troponin/tropomyosin complex significantly alter the mechanical properties of cardiac muscle. Phosphorylation of cardiac troponin I, part of the troponin/tropomyosin complex, reduces calcium affinity, which leads to increased relaxation of cardiac muscle. Because cardiac troponin I plays a central role in tuning the heart to different work demands, detailed knowledge of troponin I protein regulation is required. Our group previously detected naturally occurring antisense RNA for troponin I in human and rat hearts, and here, attempt to unravel the structure of rat cardiac troponin I antisense RNA. We performed rapid amplification of cDNA ends (RACE) experiments and discovered antisense sequences identical to a copy of the sense mRNA, which led us to conclude that the antisense RNA must be transcribed from troponin I 1 mRNA in the cytoplasm. Moreover, we isolated RNA structures comprising sense and antisense sequences in one continuous molecule. As we found no homolog structures described in the literature, we called this "hybrid RNA." Because a duplex formation was demonstrated previously we concluded that hybrid RNA is a consequence of a tight interaction between sense and antisense troponin I RNA in vivo, which we discuss in the article.
Keywords:Cardiac Troponin I, Endogenous Antisense RNA, Hybrid RNA, Sense-Antisense RNA Interaction, Animals, Rats
Source:RNA
ISSN:1355-8382
Publisher:Cold Spring Harbor Lab Press, Publications Dept (U.S.A.)
Volume:10
Number:8
Page Range:1215-1224
Date:1 January 2004
Official Publication:https://doi.org/10.1261/rna.5261204
PubMed:View item in PubMed

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