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The neuronal glycine transporter 2 interacts with the PDZ domain protein syntenin-1

Item Type:Article
Title:The neuronal glycine transporter 2 interacts with the PDZ domain protein syntenin-1
Creators Name:Ohno, K. and Koroll, M. and El Far, O. and Scholze, P. and Gomeza, J. and Betz, H.
Abstract:The glycine transporter subtype 2 (GlyT2) is localized at glycinergic axon terminals where it mediates the re-uptake of glycine from the extracellular space. In this study, we used the yeast two-hybrid system to search for proteins that interact with the cytoplasmic carboxy terminal tail region of GlyT2. Screening of a rat brain cDNA library identified the PDZ domain protein syntenin-1 as an intracellular binding partner of GlyT2. In pull-down experiments, the interaction between GlyT2 and syntenin-1 was found to involve the C-terminal amino acid residues of GlyT2 and the PDZ2 domain of syntenin-1. Syntenin-1 is widely expressed in brain and co-localizes with GlyT2 in brainstem sections. Furthermore, syntenin-1 binds syntaxin 1A, which is known to regulate the plasma membrane insertion of GlyT2. Thus, syntenin-1 may be an in vivo binding partner of GlyT2 that regulates its trafficking and/or presynaptic localization in glycinergic neurons.
Keywords:Amino Acid Sequence, Neutral Amino Acid Transport Systems, Binding Sites, Brain, Carrier Proteins, Cell Line, Cell Membrane, Glycine, Glycine Plasma Membrane Transport Proteins, Immunohistochemistry, Nerve Tissue Proteins, Neurons, Presynaptic Terminals, Protein Binding, Tertiary Protein Structure, Protein Transport, Surface Antigens, Syntaxin 1, Syntenins, Two-Hybrid System Techniques, Wistar Rats, Animals, Rats
Source:Molecular and Cellular Neuroscience
ISSN:1044-7431
Publisher:Academic Press (U.S.A.)
Volume:26
Number:4
Page Range:518-529
Date:1 January 2004
Official Publication:https://doi.org/10.1016/j.mcn.2004.04.007
PubMed:View item in PubMed

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