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Molecular analysis of the sex hormone-binding globulin gene in the rat hypodactylous mutation (Hd)

Item Type:Article
Title:Molecular analysis of the sex hormone-binding globulin gene in the rat hypodactylous mutation (Hd)
Creators Name:Liska, F. and Goesele, C. and Kren, V. and Huebner, N. and Krenova, D.
Abstract:Sex hormone-binding globulin or ABP/SHBG is an extracellular androgen and oestrogen carrier. In the rat, ABP/SHBG is secreted by Sertoli cells of the testis and is thought to regulate androgen bioavailability in the male reproductive tract. During ontogenesis, ABP/SHBG is expressed in many mesoderm-derived tissues, including interdigital mesenchyme of the developing autopodium. Shbg is thus a candidate for Hd, comprising autopodium (hand and foot) reduction and male sterility resulting from spermatogenesis impairment. Moreover, linkage mapping of Hd revealed that an intragenic marker for Shbg, D10Wox12, was non-recombinant with Hd. Sequencing of the entire coding sequence of Shbg failed to identify any variation in hypodactylous animals, distinct from two control strains. However, RT-PCR analysis revealed a significantly higher level of the Shbg transcript in hypodactylous rats compared to SHR controls. Whether Shbg expression is upregulated due to a cis-acting mutation in regulatory elements of the Shbg gene or it is a secondary result of spermatogenesis failure remains to be determined.
Keywords:Androgen-Binding Protein, Gene Expression, Limb Malformation, Linkage Mapping, Male Infertility, Rat Hypodactyly, Sequence Analysis, Sex Hormone-Binding Globulin, Animals, Rats
Source:Folia Biologica Prague
Publisher:Institute of Molecular Genetics
Page Range:63-68
Date:1 January 2004
Official Publication:http://fb.cuni.cz/Data/files/folia_biologica/volume_50_2004_2/FB2004A0010.pdf
PubMed:View item in PubMed

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