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Proliferation of human melanoma cells is under tight control of protein kinase C alpha

Item Type:Article
Title:Proliferation of human melanoma cells is under tight control of protein kinase C alpha
Creators Name:Krasagakis, K. and Lindschau, C. and Fimmel, S. and Eberle, J. and Quass, P. and Haller, H. and Orfanos, C.E.
Abstract:Exponential proliferation of human melanoma cells has been associated with low levels of protein kinase C (PKC)-{alpha}. The aim of the present study was to investigate the functional relationship between PKC-{alpha} and melanoma cell proliferation. Treatment of human melanoma cells with the selective PKC inhibitor Ro-31-8220 resulted in a significant increase of cell proliferation as measured by (3)H-thymidine incorporation and a fluorometric microassay. In addition, phosphorothioate antisense-oligodeoxynucleotides (ODNs) to PKC-{alpha} enhanced DNA-synthesis of human melanoma cells. Furthermore, microinjection and transient transfection of melanoma cells with PKC-{alpha} decreased their proliferation, as shown by the reduction of nuclear staining with the proliferation marker Ki-67. The presented data demonstrate a cause-effect relationship between PKC-{alpha} and melanoma cell growth, whereby PKC-{alpha} reversely influences the rate of cell proliferation.
Keywords:Antisense Oligodeoxyribonucleotides , Cell Division, Confocal Microscopy, Enzyme Inhibitors, Fluorescent Antibody Technique, Indoles, Melanoma, Microinjections, Protein Kinase C, Protein Kinase C-alpha, Transfection, Tumor Cell Line
Source:Journal of Cellular Physiology
Page Range:381-387
Date:June 2004
Official Publication:https://doi.org/10.1002/jcp.10434
PubMed:View item in PubMed

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