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Structure and expression of two kininogen genes in mice

Item Type:Article
Title:Structure and expression of two kininogen genes in mice
Creators Name:Cardoso, C.C. and Garrett, T. and Cayla, C. and Meneton, P. and Pesquero, J.B. and Bader, M.
Abstract:Kininogens serve dual functions by forming a scaffold for the assembly of the protein complex initiating the surface-activated blood coagulation cascade and as precursors for the kinin hormones. While rats have three kininogen genes, for mice, cattle, and humans only one gene has been described. Here, we present sequence and expression data of a second mouse kininogen gene. The two genes, kininogen-I and kininogen-II, are located in close proximity on chromosome 16 in a head-to-head arrangement. In liver and kidney, both genes are expressed and for each gene three alternative splice variants are synthesized. Two of them are the expected high and low molecular weight isoforms known from all mammalian kininogens. However, for both genes also a third, hitherto unknown splice variant was detected which lacks part of the high molecular weight mRNA due to splicing from the low molecular weight donor site to alternative splice acceptor sites in exon 10. The physiological functions of the six kininogen isoforms predicted by these findings need to be determined.
Keywords:Alternative Splicing, Gene Structure, Kallikrein-Kinin System, Animals, Mice
Source:Biological Chemistry
Publisher:de Gruyter
Page Range:295-301
Date:1 January 2004
Official Publication:https://doi.org/10.1515/BC.2004.025
PubMed:View item in PubMed

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