Nonmuscle myosin heavy-chain gene MYH14 is expressed in cochlea and mutated in patients affected by autosomal dominant hearing impairment (DFNA4)

Item Type:	Article
Title:	Nonmuscle myosin heavy-chain gene MYH14 is expressed in cochlea and mutated in patients affected by autosomal dominant hearing impairment (DFNA4)
Creators Name:	Donaudy, F. and Snoeckx, R. and Pfister, M. and Zenner, H.P. and Blin, N. and Di Stazio, M. and Ferrara, A. and Lanzara, C. and Ficarella, R. and Declau, F. and Pusch, C.M. and Nuernberg, P. and Melchionda, S. and Zelante, L. and Ballana, E. and Estivill, X. and Van Camp, G. and Gasparini, P. and Savoia, A.
Abstract:	Myosins have been implicated in various motile processes, including organelle translocation, ion-channel gating, and cytoskeleton reorganization. Different members of the myosin superfamily are responsible for syndromic and nonsyndromic hearing impairment in both humans and mice. MYH14 encodes one of the heavy chains of the class II nonmuscle myosins, and it is localized within the autosomal dominant hearing impairment (DFNA4) critical region. After demonstrating that MYH14 is highly expressed in mouse cochlea, we performed a mutational screening in a large series of 300 hearing-impaired patients from Italy, Spain, and Belgium and in a German kindred linked to DFNA4. This study allowed us to identify a nonsense and two missense mutations in large pedigrees, linked to DFNA4, as well as a de novo allele in a sporadic case. Absence of these mutations in healthy individuals was tested in 200 control individuals. These findings clearly demonstrate the role of MYH14 in causing autosomal dominant hearing loss and further confirm the crucial role of the myosin superfamily in auditive functions.
Keywords:	Amino Acid Sequence, Carrier Proteins, Cochlea, Deafness, Dominant Genes, Immunohistochemistry, Molecular Sequence Data, Mutation, Myosin Heavy Chains, Myosin Type II, Pedigree, Messenger RNA, Animals, Mice
Source:	American Journal of Human Genetics
ISSN:	0002-9297
Publisher:	University of Chicago Press
Volume:	74
Number:	4
Page Range:	770-776
Date:	April 2004
Official Publication:	https://doi.org/10.1086/383285
PubMed:	View item in PubMed

Repository Staff Only: item control page