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Single-stranded DNA bound to bacterial cold-shock proteins: preliminary crystallographic and Raman analysis

Item Type:Article
Title:Single-stranded DNA bound to bacterial cold-shock proteins: preliminary crystallographic and Raman analysis
Creators Name:Bienert, R. and Zeeb, M. and Dostal, L. and Feske, A. and Magg, C. and Max, K. and Welfle, H. and Balbach, J. and Heinemann, U.
Abstract:The cold-shock response has been described for several bacterial species. It is characterized by distinct changes in intracellular protein patterns whereby a set of cold-shock-inducible proteins become abundant. The major cold-shock proteins of Bacillus subtilis (Bs-CspB) and Bacillus caldolyticus (Bc-Csp) are small oligonucleotide/oligosaccharide-binding (OB) fold proteins that have been described as binding single-stranded nucleic acids. Bs-CspB (Mr = 7365) and Bc-Csp (Mr = 7333) were crystallized in the presence of the deoxyhexanucleotide (dT)6. Crystals of (dT) 6 with Bs-CspB grew in the orthorhombic space group C2221, with unit-cell parameters a = 49.0, b = 53.2, c = 77.0 Å. Crystals with Bc-Csp grew in the primitive orthorhombic space group P212 12, with unit-cell parameters a = 74.3, b = 64.9, c = 31.2 Å. These crystals diffract to maximal resolutions of 1.78 and 1.29 Å, respectively. The presence of protein and DNA in the crystals was demonstrated by Raman spectroscopy.
Keywords:Bacillus, Bacterial Proteins, Crystallization, Heat-Shock Proteins, Molecular Cloning, Oligodeoxyribonucleotides, Raman Spectrum Analysis, Single-Stranded DNA, X-Ray Crystallography
Source:Acta Crystallographica Section D
ISSN:0907-4449
Publisher:International Union of Crystallography (U.K.)
Volume:60
Number:4
Page Range:755-757
Date:1 January 2004
Official Publication:https://doi.org/10.1107/S0907444904002422
PubMed:View item in PubMed

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