Signal peptide cleavage of a type I membrane protein, HCMV US11, is dependent on its membrane anchor

Item Type:	Article
Title:	Signal peptide cleavage of a type I membrane protein, HCMV US11, is dependent on its membrane anchor
Creators Name:	Rehm, A. and Stern, P. and Ploegh, H.L. and Tortorella, D.
Abstract:	The human cytomegalovirus (HCMV) US11 polypeptide is a type I membrane glycoprotein that targets major histocompatibility complex (MHC) class I molecules for destruction in a proteasome-dependent manner. Although the US11 signal sequence appears to be a classical N-terminal signal peptide in terms of its sequence and cleavage site, a fraction of newly synthesized US11 molecules retain the signal peptide after the N-linked glycan has been attached and translation of the US11 polypeptide has been completed. Delayed cleavage of the US11 signal peptide is determined by the first four residues, the so-called n-region of the signal peptide. Its replacement with the four N-terminal residues of the H-2K b signal sequence eliminates delayed cleavage. Surprisingly, a second region that affects the rate and extent of signal peptide cleavage is the transmembrane region close to the C-terminus of US11. Deletion of the transmembrane region of US11 (US11-180) significantly delays processing, a delay overcome by replacement with the H-2K b signal sequence. Thus, elements at a considerable distance from the signal sequence affect its cleavage.
Keywords:	ER Subdomains, HCMV US11, Posttranslational ER Processing, Signal Sequence Cleavage, Transmembrane Anchor
Source:	EMBO Journal
ISSN:	0261-4189
Publisher:	Oxford University Press
Volume:	20
Number:	7
Page Range:	1573-1582
Date:	2 April 2001
Official Publication:	https://doi.org/10.1093/emboj/20.7.1573
PubMed:	View item in PubMed

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