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The CCAAT enhancer-binding protein alpha (C/EBPalpha) requires a SWI/SNF complex for proliferation arrest

Item Type:Article
Title:The CCAAT enhancer-binding protein alpha (C/EBPalpha) requires a SWI/SNF complex for proliferation arrest
Creators Name:Mueller, C. and Calkhoven, C.F. and Sha, X.J. and Leutz, A.
Abstract:The transcription factor CCAAT enhancer-binding protein α (C/EBPα) is a tumor suppressor in myeloid cells and inhibits proliferation in all cell types examined. C/EBPα interacts with the SWI/SNF chromatin-remodeling complex during the regulation of differentiation-specific genes. Here we show that C/EBPα fails to suppress proliferation in SWI/SNF defective cell lines after lmock-down of SWI/SNF core components or after deletion of the SWI/SNF interaction domain in C/EBPα, respectively. Reconstitution of SWI/SNF function restores C/EBPα-dependent proliferation arrest. Our results show that the anti-proliferation activity of C/EBPα critically depends on components of the SWI/SNF core complex and suggest that the functional interaction between SWI/SNF and C/EBPα is a prerequisite for proliferation arrest.
Keywords:CCAAT-Enhancer-Binding Protein-Alpha, Cell Cycle, Cell Division, Cell Line, Chromatin, Inbred BALB C Mice, Fibroblasts, Genetic Models, Genetic Vectors, NIH 3T3 Cells, Plasmids, Protein Isoforms, Reporter Genes, Retroviridae, RNA Interference, Tertiary Protein Structure, Time Factors, Transfection, Western Blotting, Animals, Mice, Rats
Source:Journal of Biological Chemistry
Publisher:American Society for Biochemistry and Molecular Biology
Page Range:7353-7358
Date:1 January 2004
Official Publication:https://doi.org/10.1074/jbc.M312709200
PubMed:View item in PubMed

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