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RP4 repressor protein KorB binds to the major groove of the operator DNA: A Raman study

Item Type:Article
Title:RP4 repressor protein KorB binds to the major groove of the operator DNA: A Raman study
Creators Name:Dostal, L. and Khare, D. and Bok, J. and Heinemann, U. and Lanka, E. and Welfle, H.
Abstract:KorB is a member of the ParB family of bacterial partitioning proteins. The protein encoded by the conjugative plasmid RP4 is part of the global control circuit and regulates the expression of plasmid genes, the products of which are involved in replication, transfer, and stable inheritance. KorB is a homodimeric protein which binds to palindromic 13 bp DNA sequences [5′-TTTAGC(G/C)GCTAAA-3′] present 12 times in the 60 kb plasmid. Each KorB subunit is composed of two domains; the C-domain is responsible for the dimerization of the protein, whereas the N-terminal domain recognizes and binds to the operator sequence (OB). Here we describe results of a Raman spectroscopic study of the interaction of the N-domain with a double-stranded model oligonucleotide composed of the palindromic binding sequence and terminal 5′-ABrU and AG-3′ bases. Comparison of the Raman spectra of the free KorB N-domain and OB DNA with the spectrum of the complex reveals large differences. KorB-N binds in the major groove of the OB DNA, and the interactions induce changes in the DNA backbone and in the secondary structure of the protein.
Keywords:Bacterial DNA, Deoxyribonucleosides, DNA-Binding Proteins, Endodeoxyribonucleases, Escherichia Coli Proteins, Exodeoxyribonucleases, Nucleic Acid Conformation, Operator Regions, Plasmids, Protein Conformation, Raman Spectrum Analysis, Recombinant Proteins, Repressor Proteins, Secondary Protein Structure, Tryptophan
Source:Biochemistry
ISSN:0006-2960
Publisher:American Chemical Society (U.S.A.)
Volume:42
Number:49
Page Range:14476-14482
Date:1 January 2003
Official Publication:https://doi.org/10.1021/bi034723h
PubMed:View item in PubMed

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