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Thermodynamic and structural analysis of peptide- and allele-dependent properties of two HLA-B27 subtypes exhibiting differential disease association

Item Type:Article
Title:Thermodynamic and structural analysis of peptide- and allele-dependent properties of two HLA-B27 subtypes exhibiting differential disease association
Creators Name:Hillig, R.C. and Huelsmeyer, M. and Saenger, W. and Welfle, K. and Misselwitz, R. and Welfle, H. and Kozerski, C. and Volz, A. and Uchanska-Ziegler, B. and Ziegler, A.
Abstract:Selected HLA-B27 subtypes are associated with spondyloarthropathies, but the underlying mechanism is not understood. To explain this association in molecular terms, a comparison of peptide-dependent dynamic and structural properties of the differentially disease-associated subtypes HLA-B*2705 and HLA-B*2709 was carried out. These molecules differ only by a single amino acid at the floor of the peptide binding groove. The thermostabilities of a series of HLA-B27 molecules complexed with nonameric and decameric peptides were determined and revealed substantial differences depending on the subtype as well as the residues at the termini of the peptides. In addition we present the crystal structure of the B*2709 subtype complexed with a decameric peptide. This structure provides an explanation for the preference of HLA-B27 for a peptide with an N-terminal arginine as secondary anchor and the lack of preference for tyrosine as peptide C terminus in B*2709. The data show that differences in thermodynamic properties between peptide-complexed HLA-B27 subtypes are correlated with a variety of structural properties.
Keywords:Amino Acid Sequence, Binding Sites, Circular Dichroism, Computer-Assisted Image Processing, Differential Scanning Calorimetry, Inborn Genetic Diseases, HLA-B27 Antigen, Peptide Fragments, Protein Conformation, Thermodynamics
Source:Journal of Biological Chemistry
ISSN:0021-9258
Publisher:American Society for Biochemistry and Molecular Biology (U.S.A.)
Volume:279
Number:1
Page Range:652-663
Date:1 January 2004
Official Publication:https://doi.org/10.1074/jbc.M307457200
PubMed:View item in PubMed

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