Early responses of human cancer cells upon photodynamic treatment monitoring by laser phase microscopy

Item Type:	Book Section
Title:	Early responses of human cancer cells upon photodynamic treatment monitoring by laser phase microscopy
Creators Name:	Roelofs, T.A. and Graschew, G. and Perevedentseva, E.V. and Rakowsky, S. and Dressler, C. and Beuthan, J. and Schlag, P.M.
Abstract:	Photodynamic treatment of cancer cells is known to eventually cause cell death in most cases. The precise pathways and the time course seem to vary among different cell types and modes of photodynamic treatment. In this contribution, the focus was put on the responses of human colon carcinoma cells HCT-116 within the first 15 minutes after laser irradiation in the presence of PhotofrinÂ« II (PII). To monitor the cell response in this early time period laser phase microscopic imaging was used, a method sensitive to changes in overall cell shape and intracellular structures, mediated by changes in the local refractive index. Laser irradiation of cells loaded with PII induced a significant reduction of the phase shifts, which probably reflects the induced damage to the different cellular membrane structures. The data suggest that even within the first 30 s after the onset of laser illumination, a significant reduction of the phase shifts can be detected. These results underline that laser phase microscopy is a suitable diagnostic tool for cellular research, also in the early time domain.
Series Name:	SPIE Proceedings Series ; 4248
Title of Book:	Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X : 20 - 21 January 2001, San Jose, USA
ISBN:	0-8194-3926-6
Publisher:	SPIE
Volume:	4248
Page Range:	163-168
Date:	20 January 2001
Official Publication:	https://doi.org/10.1117/12.424449

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