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Identification and characterization of KAT, a novel gene preferentially expressed in several human cancer cell lines

Item Type:Article
Title:Identification and characterization of KAT, a novel gene preferentially expressed in several human cancer cell lines
Creators Name:Wenzel, K. and Felix, S.B. and Flachmeier, C. and Heere, P. and Schulze, W. and Grunewald, I. and Pankow, H. and Hewelt, A. and Scherneck, S. and Bauer, D. and Hoehe, M.R.
Abstract:We describe the molecular characterization of a novel human gene on chromosome 1q23.3, termed KAT, which is highly conserved among mammals. The KAT gene spans a genomic region of approximately 1.6 kilobases and consists of 4 exons encoding a 115 amino acid protein with a molecular mass of about 12.5 kDa. The gene is expressed in several human tissues, including kidney, liver, skeletal muscle, heart, colon, thymus, spleen, placenta and lung. We identified an alternatively spliced form, lacking exon 2, in human and mouse tissues. In silico analysis of expressed sequence tags, derived from different types of human tumors, revealed another splice variant. This transcript is characterized by retention of the third intron, leading to a truncated translation product. The KAT protein is localized around the nuclear membranes. It was found to be expressed in several breast, colon and lung carcinoma cell lines, but not in normal breast epithelial cell lines. In addition, KAT protein was detected in invasive ductal carcinoma, but not in adjacent tissues. This suggests a role of this gene in tumorigenesis.
Keywords:Gene Structure, Protein Expression, Spice Variants, Tumors
Source:Biological Chemistry
ISSN:1431-6730
Publisher:de Gruyter (Germany)
Volume:384
Number:5
Page Range:763-775
Date:1 May 2003
Official Publication:https://doi.org/10.1515/BC.2003.085
PubMed:View item in PubMed

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